Purification and Properties of Turtle Heart Creatine Kinase: Role for the Enzyme in Glycolytic Control
نویسنده
چکیده
I. Adenosine triphosphate-creatine phosphotransferase (EC 2.7.3.2) from turtle (Pseudm?ys scripta) heart occurs in two major electrophoretic forms: one cytoplasmic (p1 = 6.67) and one mitochondrial (p1 = 7.37). 2i. In the forward direction (phosphocreatine + ADP + ATP + creatine), NADH actcvates the purified cytosol enzyme by lowering the K,,, for phosphocreatine almost thr e-fold. 3. From these studies it is concluded that in vivo the rising levels of NADH known to occur in the hypoxic heart of the diving turtle serve as the signal for rapid depletion of phosphocreatine and the subsequent autocatalytic activation of glycolysis. 4. The role if creatine kinase in the overall anaerobic metabolism of turtle heart is
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